It is well established that a decrease of estrogen during natural or surgical menopause (ovariectomy)
could influence female anxiety behaviour both in human and animal experiments. Several studies have
proved that exercise act as a potential therapy to reduce depression and anxiety behaviours. The current
study analyzed the effects of High-Intensity Interval Exercise (HIIE) to anxiety-related behaviours of
rats after long-term ovaries removal. Fifteen Sprague-Dawley rats (twelve weeks old) were used in this
study. All rats were divided into sham-operated, ovariectomy, and ovariectomy with HIIE (OVX
HIIE). The HIIE group was treated for 7 weeks of interval exercise that started after twelve weeks of
ovariectomized using treadmill. Anxiety related behaviour were represented by frequencies to
entrances centre arena, freezing and grooming duration on open field test. A significant decrease
(p<0.05) in the frequencies to enter open arena was detected in ovariectomized rats compared to sham
and ovariectomy with HIIE, while reversed effect has observed in freezing duration. The present results
show that HIIE may help to counteract the ovariectomized effect on anxiety-related behaviours of rats.
The HIIE may become the promising therapy to treat anxiety behaviour on menopause condition.

Keywords: Anxiety, interval exercise, ovariectomy, menopause